Unraveling Factors Affecting Micropropagation of Four Persian Walnut Varieties

Walnuts are considered recalcitrant to tissue culture, with a great genetic determinism on all stages of micropropagation; while other factors, also impact, become more complicated the reproduction newly realized varieties. In this research, holistic approach aimed determine influence genotype and nutritive formulation throughout whole cycle micropropagation four Persian walnut varieties (Juglans regia L.) was presented. During in vitro establishment it determined that besides culture medium, effect collection season likely interaction amongst factors had successful introduction genotypes. However, cultures were affected by deep decay, being necessary ethylenediamine di-2-hydroxyphenyl acetate ferric, as iron source, Phloroglucinol both Murashige Skoog (1962) corrected Driver Kuniyuki (1987) formulations. These modifications allowed stabilization cultures, maintaining thereafter steady quality. Either proliferation, rooting ex survival clones obtaining best results formulation. Finally, plants produced from acclimated high rates (75.9–91.1% for medium), depending clone medium used. Microsatellite analysis showed micropropagated maintained same profiles their corresponding donor trees. might contribute deepening understanding success walnuts, extents its influence; whereas, sets basis commercial clones.